Inhibition Test of Honey Mango Leaf Extract (Mangivera Indica L.) against the Growth of Aspergillus flavus

Abstract

Background: Aspergillus flavus is a dangerous pathogenic contaminant in agricultural commodities capable of producing highly carcinogenic aflatoxins. The escalation of resistance against synthetic chemical antifungals necessitates the discovery of safer natural alternatives. Honey mango leaves (Mangifera indica L. var. madu) accumulate potent secondary metabolites including flavonoids, tannins, saponins, alkaloids, and phenolics that manifest high antimicrobial properties. Objective: To determine the inhibitory effect of honey mango leaf (Mangifera indica L.) extract at concentrations of 80%, 70%, 60%, 50%, 40%, 30%, and 20% on the growth of Aspergillus flavus. Research Methods: This laboratory experimental design employed the disc diffusion technique using seven concentration levels (80%, 70%, 60%, 50%, 40%, 30%, and 20%) with triplicated observations. The primary metric recorded was the diameter of the inhibition zone around the paper discs. Research Results: The organic extract significantly restricted the proliferation of Aspergillus flavus across all tested ranges. The mean diameter of inhibition zones for concentrations of 80%, 70%, 60%, 50%, 40%, 30%, and 20% were measured at 39.3 mm, 33.7 mm, 31.3 mm, 26.0 mm, 24.7 mm, 20.7 mm, and 17.0 mm, respectively. Statistical trends confirmed that the inhibition area expands proportionally with the increase of extract dose. Conclusion: Honey mango leaf extract exhibits robust antifungal activities. A concentration of 50% represents the optimum threshold, yielding an inhibitory zone of 26.0 mm which demonstrates clinical parity with the commercial positive control group.